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dapi staining  (MedChemExpress)


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    Structured Review

    MedChemExpress dapi staining
    Dapi Staining, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+dapi/DAPI/pm42365506-96-13-24
    Average 98 stars, based on 182 article reviews
    dapi staining - by Bioz Stars, 2026-09
    98/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Imipramine inhibits feline infectious peritonitis virus infection by targeting host cholesterol metabolism via NPC1.
    Article Snippet: .. The procedure closely followed that of the immunofluorescence assay, with the exception that DAPI staining was replaced by staining with 0.5 mg/mL Filipin complex (MedChemExpress, USA), which specifically binds to unesterified cholesterol. ..

    Staining:

    Article Title: Imipramine inhibits feline infectious peritonitis virus infection by targeting host cholesterol metabolism via NPC1.
    Article Snippet: .. The procedure closely followed that of the immunofluorescence assay, with the exception that DAPI staining was replaced by staining with 0.5 mg/mL Filipin complex (MedChemExpress, USA), which specifically binds to unesterified cholesterol. ..

    Article Title: BMAL1 deficiency exacerbates HDM-induced asthma by promoting JUN-mediated lipid peroxidation and airway epithelial ferroptosis.
    Article Snippet: .. Paraffin sections of lung tissue (4 μm) were incubated overnight at 4 ◦C with the following primary antibodies: COX2 (1:200, 27308-1-AP, Proteintech), JUN (1:200, YM8321, Immunoway) and stained with Alexa Fluor 594 Donkey anti-Rabbit IgG (H + L) secondary antibody (A21207, Invitrogen) at room temperature for 1 h, after which nuclei were counterstained with DAPI (MedChemExpress). ..

    Article Title: Ciliatoside A Improves Klebsiella pneumoniae-Induced Pneumonia by Modulating Mitochondrial Autophagy via the SIRT1/PINK1/Parkin Pathway.
    Article Snippet: Background: Klebsiella pneumoniae (KP)induced pneumonia has a high incidence rate, and current treatment options remain limited.. The efficacy and mechanism of the novel natural compound Ciliatoside A (CA) against KPinduced pneumonia remain

    Article Title: Pyrogallic acid triggers a cascade from suppression of photosynthesis and proliferation to PCD‑like in Microcystis aeruginosa.
    Article Snippet: Pyrogallic acid (PA) is an effective allelochemical against Microcystis aeruginosa.. However, it remains unclear whether its inhibitory specificity is derived from a regulated programmed cell death (PCD)-like cascade.. A timeresolved analysis (8–96 h) of M. aeruginosa responses confirmed that 2.0 mg⋅L 1 PA induced sequential photosynthetic inhibition and proliferative arrest.

    Article Title: ARID1A loss drives osimertinib resistance by epigenetically suppressing PTEN and activating the Akt pathway in lung cancer.
    Article Snippet: Background: Osimertinib resistance poses a major therapeutic challenge in lung adenocarcinoma, and the key transcriptional regulators driving this resistance remain largely unclear.. Methods: Immunohistochemical staining was conducted to assess ARID1A protein levels in lung adenocarcinoma tissues.. An osimertinib-resistant lung adenocarcinoma cell line, PC-9 OR, was established.

    Article Title: Lipid disturbance and neuroinflammation contribute to Aflatoxin B1-linked Parkinsonism: an in vitro, in vivo, and Parkinsonism patients’ integrating evidence
    Article Snippet: Following an 8 h incubation, slides were treated with an anti-mouse secondary antibody (ab150115, Abcam, MA, USA) for 8 h at 4 °C. .. The nucleus was stained by DAPI (HY-D0814, MCE, Shanghai, China). .. Imaging visualization was carried out on an LSM980 confocal microscope (Zeiss, Jena, Germany) at 405- and 647-nm.

    Article Title: Nucleoid-associated proteins sense phage-induced genome damage to elicit abortive infection
    Article Snippet: .. Cell pellets were treated with 0.02% Triton X-100 in PBS for 5 minutes to permeabilize membranes, followed by staining with 1 μg/mL DAPI (MCE, HY-D1738) in PBS for 15 minutes in the dark to label genomic DNA. .. After washing with PBS, cells were immobilized on poly-L-lysine-coated (0.1%) glass slides and imaged using a STELLARIS STED confocal microscope (Leica Microsystems) equipped with 405 nm (DAPI) and 488 nm (GFP) laser lines.

    Incubation:

    Article Title: BMAL1 deficiency exacerbates HDM-induced asthma by promoting JUN-mediated lipid peroxidation and airway epithelial ferroptosis.
    Article Snippet: .. Paraffin sections of lung tissue (4 μm) were incubated overnight at 4 ◦C with the following primary antibodies: COX2 (1:200, 27308-1-AP, Proteintech), JUN (1:200, YM8321, Immunoway) and stained with Alexa Fluor 594 Donkey anti-Rabbit IgG (H + L) secondary antibody (A21207, Invitrogen) at room temperature for 1 h, after which nuclei were counterstained with DAPI (MedChemExpress). ..

    Article Title: Ciliatoside A Improves Klebsiella pneumoniae-Induced Pneumonia by Modulating Mitochondrial Autophagy via the SIRT1/PINK1/Parkin Pathway.
    Article Snippet: Background: Klebsiella pneumoniae (KP)induced pneumonia has a high incidence rate, and current treatment options remain limited.. The efficacy and mechanism of the novel natural compound Ciliatoside A (CA) against KPinduced pneumonia remain

    Microscopy:

    Article Title: Ciliatoside A Improves Klebsiella pneumoniae-Induced Pneumonia by Modulating Mitochondrial Autophagy via the SIRT1/PINK1/Parkin Pathway.
    Article Snippet: Background: Klebsiella pneumoniae (KP)induced pneumonia has a high incidence rate, and current treatment options remain limited.. The efficacy and mechanism of the novel natural compound Ciliatoside A (CA) against KPinduced pneumonia remain



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    Image Search Results


    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

    Journal: Biomaterials and Biosystems

    Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold

    doi: 10.1016/j.bbiosy.2026.100137

    Figure Lengend Snippet: Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

    Article Snippet: Cell culture evaluation was performed using AlamarBlue Cell Viability test (ThermoFisher), lactate dehydrogenase activity assay (LDH, Takara, Saint-Germain-en-Laye, France), ALP activity assay based on p-nitrophenol (Sigma Aldrich), calcein-AM and propidium iodide (PI) staining (Sigma Aldrich), Alexa Fluor 488 phalloidin and 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) staining (ThermoFisher).

    Techniques: Staining, Cell Culture